Introduction
The Axtra® PHY GOLD phytase product range is the newest generation phytase manufactured by IFF. It was authorised for use in the EU in 2023. The product is approved for use in the EU in all avian and porcine species. The Axtra® PHY GOLD is available in solid and liquid formulations.
Manufacturer's main claims
Reduces the anti-nutritional effects of phytate
Increased nutrient availability
Delivers market-leading thermostability
Provides accurate and effective phytase dosing
Technical specifications of Axtra® PHY GOLD 30L, 30T and 65G
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
6-phytase (EC 3.1.3.26)
Phytase activity in the product
30,000 - 65,000 FTU/g
EU approval, minimum content
All poultry except poultry for laying: 500 FTU/kg feed
All pigs: 500 FTU/kg feed
All poultry for laying: 300 FTU/kg feed
End of period of EU authorisation
6 July 2033
EU code
4a37
Production organism
Trichoderma reesei (CBS 146250)
Product storage stability
Three batches of each formulation were stored in commercial packaging for 24 months at 25°C and 60% relative humidity. After 24 months of storage, the remaining enzyme activity ranged from 85.5 - 90.7% in Axtra PHY GOLD 30L, 98.4-105.2% in Axtra PHY Gold 30T, and 95.1-107.9% in Axtra PHY Gold 65G
Storage stability in feed and premix
Storage stability in premix and feed
Three different batches of Axtra PHY Gold 30T and Axtra PHY Gold 65G were supplemented to a vitamin/mineral premix. Samples of the premix were stored for six months at 25°C in airtight plastic containers. After six months of storage, the remaining enzyme activity ranged from 90.3 - 91.8% with Axtra PHY GOLD 30T and 82.8 - 108.7% for Axtra PHY Gold 65G.
The stability of the phytase was studied in mash feed (solid product added). Feed samples were stored for three months at 25°C and 60% relative humidity. The remaining enzyme activity ranged from 83.4 - 94.5% with Axtra PHY GOLD 30T and 82.3 - 100.1% for Axtra PHY Gold 65G.
The stability of the phytase, when added to pelleted feed, was also tested using both the solid and liquid products. After three months of storage at 25C 60% relative humidity in small paper bags the residual activities were:
Axtra PHY GOLD 30L: 82.3 - 95.7%
Axtra PHY GOLD 30T: 90.4 - 105.8%
Axtra PHY GOLD 65G: 76.9 - 99.1%
Heat stability
The heat stability of each formulation was tested at different temperatures.
Axtra PHY GOLD 30T, 95°C for 30 s residual phytase activity: 89.7 - 110.7%
Axtra PHY GOLD 30L, 75°C for 30 s residual phytase activity: 80.1 - 91.7%
Axtra PHY GOLD 65G, 75°C for 30 s residual phytase activity: 81.9 - 103.9%
The content of phytase in feed pelleted at 90°C was analysed. The residual activity ranged between 80.5 and 88.5%.
Physical properties
A dusting potential test showed that Axtra GOLD 30T has an average dusting potential of 6.67 mg/m3, ranging from 0 - 20 mg/m3. The value for Axtra PHY GOLD 65G was 13.3 mg/m3.
Laser diffraction was used to determine the particle size distribution. The test showed that all particles in the T and the G products were larger than 200 µm in diameter.
Homogeneity
All three product forms were tested for their homogeneous mixing ability in the feed. Ten subsamples of mash feed supplemented with the solid forms were analysed. The liquid form was sprayed onto pellets, and ten subsamples were collected for analysis.
Coefficient of variance:
Axtra PHY GOLD 30T: 5.8 -10.2%
Axtra PHY GOLD 30L: 7.9 - 10.0%
Axtra PHY GOLD 65G: 1.4 - 1.8%
Carrier
Axtra PHY GOLD 30T: Sodium sulphate, poly-vinyl alcohol, hydrated magnesium sulphate
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment."
Manufacturer
IFF
List of references
EFSA (2022). Safety and efficacy of a feed additive consisting of 6-phytase produced by Trichoderma reesei CBS 146250 (Axtra® PHY GOLD 30L, Axtra® PHY GOLD 30T, Axtra® PHY GOLD 65G) for all poultry species and all pigs (Danisco (UK) ltd). EFSA Journal 2022;20(11):7610
EU (2023). COMMISSION IMPLEMENTING REGULATION (EU) 2023/1167 of 15 June 2023 concerning the authorisation of a preparation of 6-phytase produced by Trichoderma reesei CBS 146250 as a feed additive for all poultry species and all pigs (holder of authorisation: Danisco (UK) Ltd, trading as Danisco Animal Nutrition and represented by Genencor International B.V.). Official Journal of the European Union, L155/6-8