HiPhorius

Introduction  

HiPhorius™ is a fourth-generation phytase designed to enhance phosphorus digestibility in animal feed. It’s used primarily in poultry, swine, and aquaculture diets to break down phytate, thereby releasing usable phosphorus and improving nutrient absorption. 

HiPhorius™ was launched around early 2022, building on the legacy of previous phytase products like RONOZYME® HiPhos

Manufacturer´s main claims
Lower feed costs
Maximized animal performance
High-temperature pelleting stability
Enzyme efficiency
Minimised environmental Footprint
Measurable environmental impact

Technical specifications of HiPhorius™

EU category
Zootechnical additives

Functional group of additives
Digestibility enhancers

Active substance
6-phytase (EC 3.1.3.26)

Phytase activity in the product

Solid form: 10,000 FYT/g

Liquid form: 20,000 FYT/g

EU approval, minimum content

All poultry species for fattening or reared for laying or reared for breeding
Sows of all Suidae species

200 FYT/kg feed

All fin fish

1000 FYT/kg feed

End of period of EU authorisation

23 September 2034

EU code

4a48

Production organism

Aspergillus oryzae (DSM 33737)


Product storage stability

Shelf-Life (24 Months):

  • Solid Formulations:

    • 10°C: 0%–5% enzyme activity loss.
    • 25°C: 9%–22% enzyme activity loss.
    • 30°C: 24%–34% enzyme activity loss.

  • Liquid Formulations:

    • 10°C: 7%–9% enzyme activity loss.
    • 25°C: 32%–35% enzyme activity loss.
    • 30°C: 54%–55% enzyme activity loss.

(Stored in air-tight glass containers.)


Storage stability in premix and feed

  • Conditions: Solid forms added to premixtures at 150,000 FYT/kg, stored in plastic bags at 25°C for 6 months.
  • Result: Enzyme activity loss ranged from 25% - 33% after 6 months.

Stability in Chicken Feeds (Mash & Pelleted):

  • Conditions: Supplemented at 1,500 FYT/kg feed, stored in plastic bags at room temperature for 3 months.

Mash Feeds:

  • HiPhorius™ 10: 74%–93% enzyme activity retained.
  • HiPhorius™ 40: 104%–137% enzyme activity retained.
  • HiPhorius™ 20 L: 89%–92% enzyme activity retained.
  • HiPhorius™ 50 L: 87%–97% enzyme activity retained.

Pelleted Feeds:

  • HiPhorius™ 10: 86%–90% enzyme activity retained.
  • HiPhorius™ 40: 97%–120% enzyme activity retained.
  • HiPhorius™ 20 L: 94%–96% enzyme activity retained.
  • HiPhorius™ 50 L: 91%–99% enzyme activity retained.

Stability in Aquaculture Extruded Feeds:

  • Conditions: Sprayed at 1,000 FYT/kg feed, stored in plastic bags at room temperature for 3 months.
  • HiPhorius™ 20 L: 79%–89% enzyme activity retained.
  • HiPhorius™ 50 L: 88%–97% enzyme activity retained.


Heat stability

  • Conditions: Mixed into mash feed for chickens at 1,500 FYT/kg feed, pelleted at 80°C or 90°C (2 min mixing time).

HiPhorius™ 10:

  • 80°C: 93%–103% enzyme activity retained.
  • 90°C: 91%–116% enzyme activity retained.

HiPhorius™ 40:

  • 80°C: 79%–114% enzyme activity retained.
  • 90°C: 70%–93% enzyme activity retained.


Homogeneity

1. HiPhorius™ 10:

  • Mash Feed: Coefficient of variation = 20%.
  • Pelleted Feed: Coefficient of variation = 14%.

2. Liquid Forms (HiPhorius™ 20 L & 50 L):

HiPhorius™ 20 L:

  • Mash Feed: Coefficient of variation = < 1%.
  • Pelleted Feed: Coefficient of variation = 7%.
  • Extruded Feed (Aquaculture): Coefficient of variation = 7%.

HiPhorius™ 50 L:

  • Mash Feed: Coefficient of variation = 4%.
  • Pelleted Feed: Coefficient of variation = 5%.
  • Extruded Feed (Aquaculture): Coefficient of variation = 7%.

Note: A Lower coefficient of variation indicates a more homogeneous distribution.


Physical properties

  • Physical Form: Both are free-flowing, beige particulate products.
  • Density:
    • Hiphorius™ 10: 1,100 kg/m³ (loose) / 1,300 kg/m³ (tapped).
    • Hiphorius™ 40: 1,060 kg/m³ (loose) / 950 kg/m³ (tapped).
  • Dust Potential: Extremely low (< 0.01 mg/m³), as measured by the Stauber-Heubach method.
  • Particle Size:
    • Hiphorius™ 10: 100% of particles are larger than 250 µm.
    • Hiphorius™ 40: 100% of particles are larger than 53 µm.

  • HiPhorius™ 20 L:

    • Appearance: Light brown liquid
    • Density: 1,200 kg/m³
    • pH (1% solution in water): 7.5
    • Viscosity (25°C): 16 cP

  • HiPhorius™ 50 L:

    • Appearance: Dark brown liquid
    • Density: 1,200 kg/m³
    • pH (1% solution in water): 7.0
    • Viscosity (25°C): 40 cP


Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal breathing, eye and skin protective equipment."

Manufacturer

Novonesis/DSM

List of references

EFSA (2024). Safety and efficacy of a feed additive consisting of 6-phytase produced by Aspergillus oryzae DSM 33737 (HiPhorius™) for all poultry, all Suidae and all fin fish (DSM Nutritional Products Ltd). EFSA Journal. 2024;22:e8663.

EU (2024). COMMISSION IMPLEMENTING REGULATION (EU) 2024/2177 of 2 September 2024 concerning the authorisation of a preparation of 6-phytase produced with Aspergillus oryzae DSM 33737 as a feed additive for all poultry species for fattening or reared for laying or reared for breeding, sows of all Suidae species and all fin fish (holder of authorisation: DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp. z o.o.). Official Journal of the European Union, ELI: http://data.europa.eu/eli/reg_impl/2024/2177/oj