Introduction
The Optiphos® Plus phytase manufactured by Huvepharma was authorised for use in the EU in 2020. The product is approved for use in the EU in all avian and porcine species. The Optiphs Plus product is available in three different physical forms: coated, granulated or liquid.
Manufacturer´s main claims
Thermostable
Reliable matrix values ensure no deficiencies
Feed cost savings and reducing environmental impact
Technical specifications of Optiphos® Plus (granular, coated or liquid)
EU category
Zootechnical additives
Functional group of additives
Digestibility enhancers
Active substance
6-phytase (EC 3.1.3.26)
Phytase activity in the product
5000 FTU/g
EU approval, minimum content
250 FTU/kg feed
End of period of EU authorisation
For the below species: 6 January 2031
All poultry species
Ornamental birds
Piglets
Pigs for fattening
Sows
Minor porcine species for fattening or reproduction
EU code
4a32
Production organism
Komagataella phaffii DSM 32854 (Pichia pastoris)
Product storage stability
The product's shelf life was tested in three different batches. Product samples were stored in polyethylene bags with secondary paper bags for 24 months at 25°C (60% RH) or for six months at 40°C (75% RH).
After six months of storage, 8.9 to 13.6% of the initial phytase activity was lost.
After 24 months of storage, 7.1 to 10.4% of the initial phytase activity was lost.
Storage stability in premix and feed
Storage stability in vitamin/mineral premixes
Three batches of the coated or granulated phytase product were tested in a commercial vitamin/mineral premix containing choline chloride. Samples of the premix were stored for six months at either 25°C or 40°C.
After storage at 25°C, 9.3 to 17.4% of the granulated product's initial phytase activity was lost.
After storage at 25°C, 2.7 to 12.7% of the coated product's initial phytase activity was lost.
After storage at 40°C, 7.1 to 24.4% of the granulated product's initial phytase activity was lost.
After storage at 40°C, 9.0 to 18.6% of the initial phytase activity in the coated product was lost.
Storage stability in the final feed
The storage stability of the phytase, when added to a chicken feed consisting of maize, wheat, soybeans and sunflower, was tested. Both mash feed and pelleted feed were used in this test. The samples were stored for three months at 25°C or three weeks at 40°C.
Granulated product, mash feed
After storage at 25°C, 10.5 to 19.2% of the coated product's initial phytase activity was lost.
After storage at 40°C, 19.9 to 26.6% of the granulated product's initial phytase activity was lost.
Granulated product, pelleted feed
After storage at 25°C, 2.6 to 10.8% of the initial phytase activity in the coated product was lost.
After storage at 40°C, 14.3 to 17.3% of the granulated product's initial phytase activity was lost.
Coated product, mash feed
After storage at 25°C, 11.5 to 14.3% of the coated product's initial phytase activity was lost.
After storage at 40°C, 18.5 to 21.3% of the granulated product's initial phytase activity was lost.
Coated product, pelleted feed
After storage at 25°C, 9.8 to 11.2% of the initial phytase activity in the coated product was lost.
After storage at 40°C, 16.0 to 17.8 % of the granulated product's initial phytase activity was lost.
Heat stability
The content of phytase in feed pelleted at 90°C was analysed. The residual activity ranged between 80.5 and 88.5%.
Homogeneity
All three product forms were tested for their homogeneous mixing ability in the feed. Three subsamples of each feed used for the stability test were used. The analyses showed an average CV of 10.5% for the granulated product, 8.1% for the coated product, and 15.4% for the liquid product.
Physical appearance
The granulated product is light beige to brown. The carrier used is a mix of pregelatinised starch and wheat meal.
The coated product is also light beige to brown. Distilled monoglycerides, palm oil, wheat meal and rice hulls are used as carriers.
The liquid product is light yellow to greenish in appearance. The product uses sodium benzoate, hydrochloric acid, glycerin and water as carriers.
Handling
The European Commission states: "For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with protective equipment, including breathing, eye and skin protections."
Manufacturer
Huvepharma
List of references
EFSA (2020). Safety and efficacy of OptiPhos® PLUS for poultry species for fattening, minor poultry species reared for breeding and ornamental birds. EFSA Journal 2020; 18(6)6141
EU (2020). Commission Implementing Regulation (EU) 2020/2121 of 16 December 2020 concerning the authorisation of a preparation of 6-phytase produced by Komagataella phaffii DSM 32854 as a feed additive for all poultry species, ornamental birds, piglets, pigs for fattening, sows and minor porcine species for fattening or reproduction (holder of authorisation: Huvepharma EOOD). Official Journal of the European Union. L 426/28-31